Free Radical Scavenging Activity of Boron and Vitamin C in Nitrite-Induced Hemoglobin Oxidation Model: In vitro and in vivo Studies

Hozan Jaza  Hama Salh; Tavga Ahmed Aziz; Zheen Aorahman Ahmed; Dlivan Fattah Aziz

doi:10.54133/ajms.v5i.202

Authors

Hozan Jaza Hama Salh Department of Clinical Pharmacy, College of Pharmacy, University of Sulaimani, Kurdistan Region, Iraq
Tavga Ahmed Aziz Department of Pharmacology and Toxicology, College of Pharmacy, University of Sulaimani, Kurdistan Region, Iraq https://orcid.org/0000-0003-2742-6127
Zheen Aorahman Ahmed Department of Pharmacology and Toxicology, College of Pharmacy, University of Sulaimani, Kurdistan Region, Iraq https://orcid.org/0000-0003-4149-1566
Dlivan Fattah Aziz Department of Pharmaceutics, College of Pharmacy, University of Sulaimani, Kurdistan Region, Iraq https://orcid.org/0000-0003-0879-4899

DOI:

https://doi.org/10.54133/ajms.v5i.202

Keywords:

Boron, Methemoglobinemia, Nitrite, Oxidative stress, Vitamin C

Abstract

Objective: To investigate the dose-response relationship of the free radical-scavenging activities of boron and vitamin C in nitrite-induced hemoglobin oxidation in vitro and in vivo. Method: Different concentrations of boron and vitamin C were added to a hemolysate challenged with nitrite to induce methemoglobinemia (MetHb), and the most effective dose of boron and vitamin C was used before and after different intervals of inducing Hb oxidation, and the production of MetHb was monitored using a spectrophotometer. The effective doses of boron and vitamin C, alone and in combination, were administered to rats before challenging them with an oral dose of 100 mg/kg sodium nitrite. Results: In vitro results indicated that different concentrations of boron and vitamin C attenuated MetHb formation, with the maximum effect achieved at 0.08mg/L and 10mg/L, respectively. Moreover, when these doses were used at different time intervals, a maximum effect was achieved when added 10 min before nitrite. The in vivo results demonstrated a significant reduction in methemoglobin formation in rats treated with boron and vitamin C alone. The hematological markers were not changed except for the platelet levels, which increased in the boron-treated and combination groups. The monocyte-to-lymphocyte ratio decreased significantly in all treatment groups compared with the positive control group. Conclusion: Boron protects against Hb oxidation induced by nitrite, and a potentiated effect has been achieved with the combination of vitamin C.

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References

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