Expression of BAX and eNOS in Rabbit Pancreatic Tissues Injured by Hydrocortisone

Ahmed Fakhir Hameed; Khalida Ibrahim Noel; Mohammed Emad Shukri; Khalid Muhsin Hassan

doi:10.54133/ajms.v6i1.566

Authors

Ahmed Fakhir Hameed Department of Human Anatomy, College of Medicine, Mustansiriyah University, Baghdad, Iraq https://orcid.org/0000-0002-9400-7072
Khalida Ibrahim Noel Department of Human Anatomy, College of Medicine, Mustansiriyah University, Baghdad, Iraq https://orcid.org/0000-0001-6135-7087
Mohammed Emad Shukri Department of Anatomy, Al-Kindy College of Medicine, University of Baghdad, Baghdad, Iraq https://orcid.org/0000-0001-7864-8702
Khalid Muhsin Hassan Baghdad Teaching Hospital, Medical City, Baghdad, Iraq https://orcid.org/0009-0004-6229-1541

DOI:

https://doi.org/10.54133/ajms.v6i1.566

Keywords:

BAX, eNOS, Hydrocortisone, Immunohistochemistry, Pancreas

Abstract

Background: There have been cases of acute pancreatitis brought on by steroids, but identifying it is challenging and necessitates careful monitoring. However, according to new research, 3–5% of all cases of illness may be caused by drug-induced acute pancreatitis, making it the third most common cause. Objective: Evaluation of the effect of hydrocortisone injections on pancreatic structure immunohistochemically using BAX and eNOS markers. Methods: White New Zealand female rabbits weighing between 1.2 and 1.5 kg were used, and they were given free access to food. The rabbits were split into six groups, with five animals in each group receiving intramuscular hydrocortisone injections for 14 and 21 days, respectively, at a dose of 5 mg and 20 mg/kg for short and long durations, and two control groups. Results: There was an increase in weight in both long-duration groups (GL1 and GL2) after week 2 of injection when compared to both control and short-duration groups. There was a highly statistical difference in the expression of BAX in both short- and long-duration groups compared to the control group, and there was also a decrease in the expression of BAX when duration increased. Similarly, there was a highly statistical difference in the expression of eNOS in both the GS and GL groups when compared to the control group. Conclusions: The pancreas can be injured by high and low doses of hydrocortisone if used for more than 2 weeks.

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