Role of miRNA 199a-5p Expression in Iraqi Women with Breast Cancer

Arwa Mohammed Salih; Ismail Hussein Aziz; Forat Yahya Mohsin

doi:10.54133/ajms.v5i1S.308

المؤلفون

Arwa Mohammed Salih Forensic DNA Center for Research and Training, Al-Nahrain University, Jadriya, Baghdad, Iraq https://orcid.org/0009-0005-8968-4289
Ismail Hussein Aziz Institute of Genetic Engineering and Biotechnology for Postgraduate Studies, University of Baghdad, Baghdad, Iraq https://orcid.org/0000-0003-0263-3357
Forat Yahya Mohsin Oncology Teaching Hospital, The Medical City, Baghdad, Iraq

DOI:

https://doi.org/10.54133/ajms.v5i1S.308

الكلمات المفتاحية:

Breast cancer، Epithelial-mesenchymal transition، Gene expression، miRNA-199a-5p، N-cadherin، RT-PCR

الملخص

Background: Elevated levels of microRNAs have been linked to breast cancer and other types of cancer. It has been discovered that miRNA-199a-5p overexpression contributes to the epithelial-mesenchymal transition (EMT) in breast cancer. Objective: To examine the expression of microRNA 199a-5p in tissues from both benign and malignant breast cancers. Methods: We use 50 FFPE tissue samples divided into two groups based on their histology and cytological characteristics. The first group comprised 25 samples classified as benign, while the second group comprised 25 samples classified as malignant. To calculate the expression levels of miRNA-199a-5p, we use a real-time RT-PCR method based on SYBR green. Results: It was found that the folding value of miRNA-199a-5p in malignant tumors was 2.908 compared to benign tumors (2.027). However, the statistical significance of these differences was not significant. Conclusion: Depending on the specific context and molecular targets, miRNA-199a-5p plays a dual role in breast cancer development, acting as both an oncogene and a tumor suppressor.

التنزيلات

بيانات التنزيل غير متوفرة بعد.

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