Preparation and Effects of Manganese Oxide Nanoparticles Against Quinolone-Resistant Bacteria Isolated from Hospital Wastewater

Mohammed Fadhil AboKsour; Mohammed Faraj Al-Marjani; Ahmed Mahdi Rheima

doi:10.54133/ajms.v6i2.728

المؤلفون

Mohammed Fadhil AboKsour Department of Microbiology, College of Sciences, Mustansiriyah University, Baghdad, Iraq https://orcid.org/0000-0002-5577-4009
Mohammed Faraj Al-Marjani Department of Microbiology, College of Science, Mustansiriyah University, Baghdad, Iraq
Ahmed Mahdi Rheima Department of Chemistry, College of Science, Mustansiriyah University, Baghdad, Iraq https://orcid.org/0000-0003-3533-3393

DOI:

https://doi.org/10.54133/ajms.v6i2.728

الكلمات المفتاحية:

Antibacterial activity، Antibiofilm effect، Bacterial resistance، MnO2NPs

الملخص

Background: The widespread administration of quinolones may have led to an increase in bacterial resistance development. Objective: To synthesize and characterize manganese oxide nanoparticles (MnO2NPs) and evaluate their effects on the viability and biofilm formation of quinolone-resistant gram-positive and negative pathogenic bacteria. Methods: We prepared MnO2NPs using the photo-irradiation method and recorded their characteristics using XRD, TEM, and SEM. Staphylococcus aureus, Streptococcus pneumonia, Escherichia coli, Klebsiella pneumonia, and Pseudomonas aeruginosa were collected from the wastewater of Baghdad hospitals during the period from July 3^rd to July 14^th, 2023. We used the VITEK2 system to confirm and identify all of the isolates. We conducted biofilm formation and antibiotic susceptibility tests using nalidixic acid, ciprofloxacin, norfloxacin, aztreonam, levofloxacin, and ofloxacin, and also detected the qnrA and qnrB genes. Finally, we evaluated the effects of the prepared nanoparticles on the viability and biofilm formation of bacterial isolates. Results: MnO2NPs characterizations showed a diffraction peak at 2θ values with 21 nm average sizes; qnrA and qnrB genes were found in three and four isolates, respectively; and significant effects of MnO₂NPs against viability and biofilm formation were recorded. Conclusions: The synthesized nanoparticles have antibacterial and anti-biofilm activities against a variety of bacteria possessing qnr genes. Even multi-resistant bacterial isolates have the potential to be strong antimicrobial agents against these pathogens.

التنزيلات

بيانات التنزيل غير متوفرة بعد.

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